Movie: Nadinath Nillegoda '04

Watch time-lapse movies or focus through thick specimens on the confocal microscope!

NOTE: These movies are in the Macromedia Flash format. Most web browsers already have the needed plugin, but if you can't see the movies, click here to download the plugin.

 
 

Image: Nadinath Nillegoda '04

Bovine pulmonary arterial endothelial cells which have been dual labeled with a red stain for actin microfilaments (Rhodamine Phalloidoin) and a green fluorescent antibody against microtubules. These are the same cells as shown above, however the microtubules are found dispersed in the cytoplasm where they form an important part of the cytoskeleton.
 
 

Image: Susan Schiffer '03

Basal region of an Arabidopsis root expressing GFP targetted to the vacuolar membrane. Obtained from ABRC stock center, stock number CS84727, as part of the Ehrhardt GFP collection.
 
 

Image: Susan Schiffer '03

Arabidopsis leaf epidermis expressing GFP fused to an aquaporin that localizes to the plasma membrane (ABRC stock no. CS84758). A stoma is readily apparent at the lower right of the image. The "jigsaw" cells are known as pavement cells and make up the bulk of the cells of the epidermis.
 
 

Image: Nadinath Nillegoda '04

Bovine pulmonary arterial endothelial cells which have been dual labeled with a green fluorescent antibody against tubulin and a red antibody against actin. Since the mitotic spindle is composed of tubulin, it stains brightly during mitosis. The chromosomes are not visible, but would be at the center of this image.
 
 

Image: Susan Schiffer '03

Arabidopsis root expressing GFP targetted to an unknown subcellular location (ABRC stock no. CS84729). Some appears to be localized to the endoplasmic reticulum. A root hair, formed by localized tip growth of an epidermal cell, is apparent in the middle of the image.
 
 

Image: Nadinath Nillegoda '04

Bovine pulmonary arterial endothelial cells which have been dual labeled with a green stain for actin microfilaments (FITC-Phalloidoin) and a red stain for mitochondria. The stain for actin is actually derived from a toxic compound found in mushrooms. The stain for mitochondria is interesting because it only becomes fluorescent when the stain is activated by enzymes which reside in the mitochondria. For this reason, only the mitochondria appear red, not the other organelles.

Last modified July 18, 2002 by Chris Wolverton